Molecular Biology Biotechnology

MOLECULAR BIOLOGY & BIOTECHNOLOGY DIVISION

Objective: Assessment, characterization and documentation of fish genomic resources.

Thrust Areas:

  • Genomic, transcriptomic and proteomic studies of commercially important species
  • Physical mapping of genes
  • Genetic characterization and population genomics
  • Reproductive biotechnology
  • Fish cell biotechnology
  • Development and maintenance of genomic resources and databases

Salient Achievements:

  • Developed a non-invasive technique for rapid extraction of DNA from fish scales
  • Generated DNA barcodes over 500 Indian fish species
  • Generated chromosome profiles of over 70 fish species
  • Physical mapping of rRNA genes in17 fish species using FISH
  • Established National Repository of Fish Cell Line and maintaining 50 fish cell line in the NRFC
  • Developed cell lines from 10 fish species
  • Developed online databases, viz. Fish Karyome, Fish Barcode Information System, Fish Microsatellite and Fish Mitogenome
  • Evaluated genotoxicity of aquatic pollutants using cytogenetic and molecular assays using in vivo and in vitro systems
  • Gene expression profiling in fishes following heavy metal and pesticide exposure
  • Generated protein profile in Labeo rohita
  • Molecular modelling,and docking of virus and shrimp proteins involved in white spot disease and screening of potential inhibitors
  • Gene expression profiling in fishes following heavy metal and pesticide exposure

Facilities available in the Division:

  • High performance computing (HPC) system with CLC Bio and Discovery Studio software
  • ABI sequencer 3500
  • Fluorescent imaging system
  • RT-PCR
  • Capillary Electrophoresis system
  • 2D-Electrophoresis system and Comet image analysis system.